COVID-19 PCR Detection Kit

Features:

• Highly specific for the ORF1ab and N target markers recommended by the World Health Organization (WHO) and the Centers for Disease Control and Prevention (CDC) in the United States.
• Ready-to-use reaction kit that includes MasterMix, Primers/Probes, and Internal Positive Control.
• Can be used with samples collected from sputum and naso/ oropharyngeal swabs.
• Compatible with standard RT-qPCR machines (Applied Biosystems 7500, QuantStudio 7 Flex, or Bio-Rad CFX96 recommended).

The Covid 19 RT-PCR Detection Kit is a one-step RT-PCR Kit for the detection of SARS-CoV2-specific RNA in specimens including serum from suspected patients. The kit contains SARS-CoV-2-specific primer and probe sets designed by comparing all available sequences of other coronaviruses that allows both transcription and subsequent PCR amplification in a single reaction. An internal control for human RNA species will be provided with the kit to identify possible PCR inhibition and assess the reliability of the PCR reaction.

The limits of detection for this kit are:
• 10 copies/reaction for the ORF1ab region
•10 copies/reaction for the N-target region

This Assay is validated on the Applied Biosystems® 7500 Fast and 7500 Real-Time PCR system.