Our Gene Vectors

Below is a listing of vectors available in-house. Please enquire about vectors other than listed below, as we may or may not carry them in-house and would need to be shipped to us for subcloning projects. Please inquire at [email protected] and we will be happy to assist you.

In-house Vectors

pUC57m (No NdeI)pBluescript II SK-

Specialty Vectors

pGL4.17[luc2/ Neo]pGL4.19[luc2CP Neo]

More vectors available than listed. Please enquire

How do I ship my own vectors to NBS for cloning?

Please send two tubes each containing 4.0ug of each plasmid DNA, with your project number as a memo, to

NBS Biologicals Ltd
14 Tower Square
Huntingdon
PE29 7DT

Once shipping has been arranged, please inform [email protected] of the tracking number.
Please contact us at [email protected] if you have any questions or concerns regarding shipping.

1

pUC57-Amp

Sequence map

“Cloning vector with an ampicillin resistance marker, suitable for generating ExoIII deletions.” The pUC57’s MCS contain 6 restriction sites with 3’-ends that are resistant to ExoIII.
Source: www.snapgene.com

2

pUC57-Kan

“Cloning vector with a kanamycin resistance marker, suitable for generating ExoIII deletions.”

3

pUCSP

Sequence map

Source: www.addgene.org

4

pBluescript II SK+

Sequence map

“Standard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA.”
Source: www.snapgene.com

5

pET-21A

Sequence map

“Bacterial vector for inducible expression of N-terminally T7-tagged proteins.”
Source: www.snapgene.com

6

pET-28B

Sequence map

“Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site.”
Source: www.snapgene.com

7

pBR322

Sequence map

Source: www.snapgene.com

8

pBluescript II KS+

Sequence map

Source: www.snapgene.com

9

pBluescript II SK-

Sequence map

Source: www.snapgene.com

10

pUCm-T

Sequence map

Source: www.biobasic.com

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