Blue Gravity Column

Blue Gravity Column: The carbohydrate nature of the agarose base of Blue sefinoseTM 6 fast flow provides a hydrophilic and chemically favorable environment for coupling and excellent chromatographic properties. The dye ligand, Cibacron Blue F3G-A, is covalently attached to the Blue sefinoseTM 6 fast flow matrix via the triazine part of the dye molecule. Cibacron Blue F3G-A shows certain structural similarities to naturally occurring molecules like the co-factors NAD+ and NADP+ which enables it to bind strongly and specifically a wide range of proteins, including kinases, dehydrogenases and other enzymes requiring adenylyl-containing substances. Of the enzymes currently catalogued, approximately a third require a nucleotide coenzyme, suggesting the potential application of ligands such as Cibacron Blue F3G-A is extremely wide. The use of immobilised dyes in place of immobilised nucleotides has a number of advantages. Firstly, dye ligands have greater versatility, which allows the purification of several proteins without tedious preparation of a range of gels. Secondly, the chemical stability of the adsorbent allows its continued re-use. Thirdly, loss of activity through storage is not a problem. We also offer pre-packed, ready-to-use 1ml gravity columns for preparative affinity chromatography. Fast, simple and easy separations are provided by the combination of a specially designed column and a high performance affinity medium. The resin is very versatile and particularly suitable for the isolation and purification of albumin, interferon, a broad range of nucleotide requiring enzymes, a2-macro-globulin, coagulation factors, and nucleic acid binding proteins. The removal of albumin is a particularly important application since excess amounts of albumin can disturb the results of several tests.