Gravity Column De-Salting

Gravity Column De-Salting: Bio Basic’s Desalting Gravity Columns is a ready-to-use desalting columns for separating larger molecules from small molecules and buffer components. More specifically, molecules greater than 5,000 MW will elute in the void volume, separating them from smaller molecular weight buffer salts and reagents. Buffer-Exchange can also be achieved by first equilibrating a column with the desired buffer, then applying sample. Molecules greater than 5,000Da will then be exchanged into the desired buffer. These columns are pre-packed with cross-linked dextran and display excellent chromatographic properties and have good rigidity for easy handling and good flow properties. The Gel is stable, easy to use, and provides excellent sample recoveries. The gel can withstand water, salt solutions, organic solvents, alkaline and weakly acidic solutions. It is stable to heat and can be autoclaved dry or heated at 120°C in solution of neutral pH for 30 minutes without affecting its chromatographic properties. The desalting process or buffer exchange can therefore be performed in less than one hour. The column displays unique stop-flow characteristics which prevents drying of the gel and loss of precious samples. Recoveries of 90% or greater can be expected, assuming that the desalting buffer does not enhance nonspecific binding between the protein and the matrix.