Chloroform:isoamyl alcohol (24:1)

Chloroform:isoamyl alcohol (24:1): trichloride1This product is designated as ACS Reagent grade,and meets the specifications of the American Chemical Society (ACS) for reagent chemicals. Chloroform is a widely used organic solvent in biochemistry and molecular biology. Procedures have been described for the use of chloroform in λ plaques storage, for λ cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for β-galactosidase activity. Chloroform has been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA.2 A protocol describes the use of chloroform in a high-performance thin-layerchromatography protocol for sphingomyelin analysis.3 A protocol has been described for the use of chloroform/methanol for the isolation of cardiolipids from Geobacillus tearothermophilus and their subsequent MS analysis.4 The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure.5 The use of chloroform/2-butanol for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry has been reported.6The analysis in chloroform of synthetic protein core mimetics has been described.7 The solubility of various noncovalent metallodendrimers containing hydrophobic