The EZ-10 Total RNA Mini-Preps Kit allows efficient purification of total RNA from various samples, total RNA is easily purified from animal or human cells and tissues using a simple spin format.
Biological samples are first lysed and homogenized in denaturing guanidine-containing buffer (Buffer RLT), which immediately inactivates DNases and RNases as well as proteases to ensure isolation of intact RNA. RNA in the whole homogenate is selectively absorbed on EZ-10 Spin Column, all protein and other components are removed in the flow-through. Remaining contaminants and salts are efficiently washed away. Purified RNA is eluted in RNase-free water has OD260/OD280 ratios of 1.9-2.1 and is ideal for use in most downstream applications including Northern blotting, RT-PCR, Quantitative PCR, Poly (A) RNA selection and Array analysis.
