FFPE Protein Extraction Kit

FFPE Protein Extraction Kit: In vitro cultures may not represent true differenal protein expression paerns, as a result, in vivo, formalin‐fixed, paraffin‐embedded ssues represent a comprehensive source of protein expression profiles especially in clinical studies or studies of diseases such as cancer. These samples enable the course of disease — e.g., before and aer therapy — to be examined . FFPE Protein Extracon Kit provides opmized condions for extracng total protein from FFPE or formalin‐fixed issue secons. Aer rapid 5‐minute de‐paraffinizing by de‐paraffinizaon reagent, the samples are incubated in an opmized extracon buffer at two different temperatures in a process that reverses formalin crosslinking and untangles the protein molecules. Aer a centrifugaon step, the supernatant containing the released proteins is precipitated, recovered and can be used for Western Blot , SDS‐PAGE and other applicaons. The kit can perform 50 mini‐preps.