GST-Sefinose Resin

GST-Sefinose Resin: The glutathione ligand is coupled via a 10-carbon linker to highly cross-linked 6% agarose. The coupling of Glutathione Sefinose™ resin is optimized to provide high binding capacity and one-step purification of glutathione S-transferase (GST) tagged proteins expressed from pGEX series vectors, as well as other glutathione S-transferases and glutathione binding proteins. GST-tagged proteins can be purified directly from pre-treated bacterial lysates using Glutathione Sefinose resin. The tagged proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and function. The Glutathione sefinose resin is an excellent choice for high performance purifications. Glutathione sefinose resin is also available in conveniently pre-packed 1-ml, 5ml and 10-ml column sizes.If removal of the GST moiety (a naturally occurring protein with Mr 26 000) is required, the tagged protein can be digested with appropriate site-specific protease while bound to Glutathione Sefinose or, alternatively after elution. Cleavage of GST-tagged protein bound to resin eliminates the extra step of separating the released protein from GST. The cleaved target protein is eluted using binding buffer.