India Ink Stain Solution

India Ink Stain Solution: India ink (Pelikan 17 black) is used to stain electro‐blo􀀽ed proteins on blot transfer membranes. Transferred proteins (>5 ng/band) appear as black bands on a gray background. Proteins are stained in the India ink solu􀀲on a􀀷er electroblo􀀸ng from one‐dimensional or two‐dimensional polyacrylamide gels to blot membranes such as polyvinylidene difluoride (PVDF) and nitrocellulose membranes. India ink staining is not suitable for nylon membranes or hydrophobic proteins on membrane. The solu􀀲on is provided as a 5x Strength solu􀀲on in PBS.