Pfu DNA Polymerase, 5u/ul

Pfu DNA Polymerase, 5u/ul: Product Description:
Cloned Pfu DNA polymerase is isolated from the hyperthermophilic marine archaebacterium. Pyrococcus furiosus. The mulfunconal thermostable enzyme possesses both 5’‐ to 3’‐ DNA polymerase and 3’‐ to 5’‐ exonuclease acvity, which results in a 12‐fold increase in fidelity of DNA synthesis over Pfu DNA polymerase. Cloned Pfu DNA Polymerase has a temperature opmum between 72°C and 78°C remains > 95% acve following one hour incubaon at 95°C.
10 x Reaction Buffer: 200 mM TrisHCl ( pH 8.8) 100 mM KCl 100 mM (NH4)2 SO4 20 mM MgSO4 1% Triton X‐100 1 mg / ml nuclease‐free bovine serum albumin (BSA)
Unit Activity: One unit of acvity is the amount of cloned Pfu DNA polymerase required to incorporate 10nM of [H] TTP into an acid‐insoluble form in 30 minutes at 72°C.
Contaminants: Cloned Pfu DNA polymerase is tested extensively for the absence of detectable nonspecific nuclease acvity and DNA contaminaon.
Shipping Conditions: Cloned Pfu DNA polymerase is shipped in gel package. But remains full acvity at room temperature.