Pfu DNA Polymerase, 5u/ul: Pfu DNA Polymerase, 5u/ul
Product Description:
Cloned Pfu DNA polymerase is isolated from the hyperthermophilic marine archaebacterium. Pyrococcus furiosus. The multinational thermostable enzyme possesses both 5’‐ to 3’‐ DNA polymerase and 3’‐ to 5’‐ exonuclease activity, which results in a 12‐fold increase in fidelity of DNA synthesis over Pfu DNA polymerase.
Cloned Pfu DNA Polymerase has a temperature optimum between 72°C and 78°C remains > 95% active following one hour incubation at 95°C.
10 x Reaction Buffer:
200 mM TrisHCl ( pH 8.8)
100 mM KCl
100 mM (NH4)2 SO4
20 mM MgSO4
1% Triton X‐100
1 mg / ml nuclease‐free bovine serum albumin (BSA)
Unit Activity:
One unit of activity is the amount of cloned Pfu DNA polymerase required to incorporate 10nM of [H] TTP into an acid‐insoluble form in 30 minutes at 72°C.
Contaminants:
Cloned Pfu DNA polymerase is tested extensively for the absence of detectable nonspecific nuclease activity and DNA contamination.
