pUCM-T Cloning Vector Kit

pUCM-T Cloning Vector Kit: The T-Vector PCR Product Cloning Kit is suitable for cloning of PCR products with additional A at 3’ end. The special ligation system provided by the kit enables customs to finish ligation in 1-2 hour. pUCm-Tvector of our company isdesign for simplifing cloning of PCR products. Many thermal stable DNA polymerase, PCR products amplified by DNApolymerase such as Taq,Tth DNA produce additional A at 3’ end which could be easily ligated to T vector with additional T. pUCm-T of our company is a kind of noval pUC derivative T vector, the mutiple restrict sits with most of them single site and adjusted b-galatose reading frame make it easily for screening target clone through blue and white plaque. Specially designed two Pst I sites beside inserted fragments make it easy for screening target clone by Pst I digestion, at same time inserted fragments also could be screened by cheap and efficient restrict enzymes such as EcoR I and Hind III double digestion. Inserted fragmentsalso could be sequencedusing universal primers M13 and T7 promoterprimer. In vitro transcription could be processed through site of T7 RNA polymerase promotor in pUCm-T.