SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures.
This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (the final solution is a 20x).
The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.
Application:
Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
• Preparing nucleic acids for hybridization
• Hybridization buffer for Northern and Southern blots
• Washing buffer for Northern and Southern blots
Features:
• Multi-purpose use in hybridizations
• Easily reconstituted and diluted to any concentration needed
Components:
1PK of SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M sodium chloride.
Instructions:
Dissolve 1PK powder in molecular biology grade to final volume 1L at 25°C or above to yield 1L of 20X SSC Buffer.
