Sudan black B

Sudan black B: Sudan Black B was found to eliminate lipofuscin-like autofluorescence in mammalian neural tissue without adversely affecting other fluorescent labels. It was also found to reduce autofluroescence in archival formaldehyde-fixed paraffin-embedded myocardium tissue. Sudan Black B was used to detect native and oxidized low density lipoproteins (LDLs) after separation by capillary isotachophoresis (CITP). It has been used for staining fat in animal tissues and in bacteria (Burdon’s method), as well as the lipid portion of lipoprotein in polyacrylamide gel electrophoresis. It has also been used for staining lipofuchsin.