Yeast Protein Lysis Buffer, 1x Strength Solution

Yeast Protein Lysis Buffer, 1x Strength Solution: Yeast protein Lysis Buffer uses a mild detergent formulation for protein extraction that is superior to the classical methods of protein isolation from yeast. In studies with Pichia pastoris, yields of soluble protein typically exceed those achieved by the standard glass bead disruption method. The unicellular nature of yeast, combined with its ability to perform post-translational modifications that closely mimic processes in higher eukaryotes, have made them important research tools and recombinant expression systems. The yeast cell is difficult to lysis, this is because yeast cells possess very complex proteinaceous cell wall that provides rigidity to the weak plasma membrane. Techniques for yeast cell lysis often involve harsh mechanical treatment while using strong reducing agents, chemicals and pH and temperature extremes. The popular glass bead lysis protocol requires special equipment and must be performed at 4°C. The low yields of protein commonly obtained with this technique are the result of denaturation and proteins nonspecifically binding to the glass beads. However, Yeast Protein Lysis Buffer uses a simple room temperature protocol that can be completed in 20 minutes and require no special equipment. The lysis buffer is supplied with PMSF, protease inhibitors and DTT to improve protein stability, to maintain protein integrity and extraction efficiency. Depending on the required downstream applications, additional agents such as phosphatase inhibitor and chelating agents may be added into the kit. 20ml is sufficient for 20x 50mg wet weight of the sample.