Protein Stain-EZ B, Reversible Zinc Stain Kit

Proetin Stain-EZ B, Reversible Zinc Stain Kit: The Reversible Zinc Stain Kit is designed for rapid detection of proteins fractionated by PAGE (native gels or SDS denatured gels).The stain is based on the interaction of Zinc ions with polyacrylamide and proteins. The stain works by depositing zinc metal precipitate in the gel, which turns the gel opaque white, while the SDS coating on the proteins prevents the stains from binding to the proteins. A negative image is produced; clear protein bands are detected against a semi-opaque white polyacrylamide background. Protein bands are visualized in as little as 10-15 minutes. The sensitivity of the Reversible Zinc Stain is 8-12ng and does not interfere with the electroelution of proteins or alter their biological properties. Gels stained with the Reversible Zinc Stain can be erased in 15 minutes before the transfer or electroelution of proteins. This stain works with native as well as SDS denatured gels and gels containing Glycine, Tricine and a variety of primary-amine containing buffers. The kit is sufficient for 10 minigels.