Protein Stain-EZ C, Reversible Copper Stain Kit

Reversible Copper Stain Kit (Protein Stain-EZ C): The Reversible Copper Stain Kit is designed for the rapid detection of proteins fractionated by SDS-PAGE. The stain is based on the interaction of copper ions with polyacrylamide and proteins. The stain works by depositing a copper metal precipitate in the gel, which turns the gel opaque green, while the SDS coating on the proteins prevents the stains from binding to the proteins. A negative image is produced; clear protein bands are detected against a semi-opaque blue polyacrylamide background. Protein bands are visualized in as little as 30 minutes. The sensitivity of the Reversible Stain is 5-12ng. Staining does not interfere with the electroelution of proteins or alter their biological properties. Gels stained with the Reversible Copper Stain kit can be Erased in 15-20 minutes before the transfer or electroelution of proteins. This stain can also be suitable for native gels or gels containing Tricine or Glycine. The kit is sufficient for 10 minigels.